| John Huston |
| Humphrey Bogart | .... | Sam Spade |
| Mary Astor | .... | Brigid O'Shaughnessy |
| Gladys George | .... | Iva Archer |
| Peter Lorre | .... | Joel Cairo |
| Barton MacLane | .... | Detective Lieutenant Dundy |
| Sydney Greenstreet | .... | Kasper Gutman |
| Ward Bond | .... | Detective Tom Polhaus |
| Jerome Cowan | .... | Miles Archer |
| Elisha Cook Jr. | .... | Wilmer Cook |
| James Burke (I) | .... | Luke |
| Murray Alper | .... | Frank Richman |
| John Hamilton (I) | .... | Attorney Bryan |
| Emory Parnell | .... | Ship's Mate |
| Robert Homans | .... | Policeman |
| Creighton Hale | .... | Stenographer |
| Charles Drake | .... | Reporter |
| William Hopper | .... | Reporter |
| Hank Mann | .... | Reporter |
| Jack Mower | .... | Announcer |
| Walter Huston | .... | Captain Jacobi |
| Lee Patrick | .... | Effie Perine |
| Dashiell Hammett | (novel) | ||
| John Huston |
| Arthur Edeson |
| Adolph Deutsch |
| Orry-Kelly |
| Thomas Richards |
| Henry Blanke | (associate) |